Prometheus: Life and Death is a four-issue limited comic book series that was first published by Dark Horse Comics from June-September 2016. It was also published simultaneously in the digital format via Dark Horse Digital. The story was written by Dan Abnett, illustrated and inked by Andrea Mutti, colored by Rain Beredo, lettered by Michael Heisler and edited by Freddye Miller, Randy Stradley and Kevin Burkhalter, with cover art by David Palumbo. Sachin Teng and Dave Dorman also provided variant covers for the first issue.

Prometheus: Life and Death formed the Prometheus component of Dark Horse's franchise-crossing Life and Death event in 2016/2017, and was released in conjunction with Predator: Life and Death, Aliens: Life and Death and Alien vs. Predator: Life and Death. The four series all form one continuous story and all were written by Abnett. Prometheus: Life and Death takes place after Predator: Life and Death.

In the Prometheus comics line, Prometheus: Life and Death was preceded by Prometheus: The Complete Fire and Stone, and will be followed by Prometheus: Life and Death - Final Conflict.